1
صنایع غذایی::
گونه های اکسیژن واکنش پذیر
(NO), reactive oxygen species (ROS) and PGE2, and also upregulates the surface level of glycoproteins (co-stimulatory molecules CD80 and CD86, adhesion molecules) (Bresnihan, 1999; Burmester et al.
< Example 6 NBG-CHCl in TNF-α-stimulated Vascular Endothelial Cells 3 Effect of ROS on the production of reactive oxygen species in vascular endothelial cells
To analyze the effect of NBG-CHCl 3 on the regulation of the production of reactive oxygen species (ROS) produced in vascular endothelial cells by TNF-α stimulation, 2'7'-dichlorodihydrofluorescin diacetate (DCF-DA) Immunofluorescence staining was used.
It was encapsulated in Vectashield and analyzed for ROS generation using a fluorescence microscope.
As a result, the expression of ROS increased by TNF-α was completely reduced by NBG-CHCl 3 .
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2
عمومی::
گونههای اکسیژن واکنشگر
, interleukins; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; M-CSF, macrophage colony-stimulating factor; MCP-1/5, monocyte chemoattractant protein-1/5 (CCL2/CCL12); MHCII, major histocompatibility complex class II; MIP- 1α/β, macrophage inflammatory protein-1 alpha/beta (CCL3/CCL4); MNCs, mononuclear cells; MMPs, matrix metalloproteinases; MSCs, mesenchymal stromal cells; NF-κβ, nuclear factor kappa beta; PAMPs, pathogen-associated molecular patterns; PDGF, platelet-derived growth factor; PGE-2, prostaglandin E-2; ROS, reactive oxygen species; TAMs, tumor-associated macrophages; TGF- β1, transforming growth factor beta 1; TNF-α, tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor.
Neutrophils produce high levels of reactive oxygen species (ROS), proteases and pro-inflammatory cytokines to sanitize the wound.
Pro-inflammatory macrophages produce nitric oxide, ROS, IL-1, IL-6, and TNF-α.
M1 macrophages express CD86, and produce high levels of (ROS) and pro-inflammatory cytokines, such as interleukins 1 and 6 (IL-1, IL-6), TNF- α, and IFN-γ.
In vitro studies found that ROS, rather than growth factors or cytokines (KGF, EGF, TNF-α), directly stimulated HO-1 expression.
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